Publications

Johns Hopkins University

DateArticle JournalPDF
1/15/2025
Investigating subpopulation dynamics in clonal CHO-K1 cells with single-cell RNA sequencingJournal of Biotechnology***
1/5/2025Mitigating Membrane Biofouling in Protein Production with Zwitterionic PeptidesLangmuir***
12/9/2024Modeling Solubilities for Amino Acids in Water as Functions of Temperature and pHIndustrial & Engineering Chemistry Research***
9/1/2024Ala-Cys-Cys-Ala dipeptide dimer alleviates problematics cysteine and cystine levels in media formulations and enhances CHO cell growth and metabolismMetabolic Engineering***
8/1/2024A semi-empirical equation modeling long-range contributions to the activity coefficients of individual ions at high ionic strengthsJournal of Molecular Liquids***
5/24/2024Investigating Subpopulation Dynamics in Clonal CHO-K1 Cells with Single-Cell RNA SequencingBioRxiv***
5/1/2024Elucidating uptake and metabolic fate of dipeptides in CHO cell cultures using 13C labeling experiments and kinetic modelingMetabolic Engineering***
4/1/2024Data-driven and physics informed modeling of Chinese Hamster Ovary cell bioreactorsComputers & Chemical Engineering***
3/18/2024Comprehensive stable-istope tracing of glucose and amino acids idenitifies metabolic by-products and their sources in CHO cell culturePNAS***
10/1/2023Some of the variables, some of the parameters, some of the times, with some physics known: Identification with partial informationComputers & Chemical Engineering***
7/21/2023Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturingBiotechnology and Bioengineering***
5/6/2023Chemical inhibitors of hexokinase-2 enzyme reduce lactate accumulation, alter glycosylation processing, and produce altered glycoforms in CHO cell culturesBiotechnology and Bioengineering***
4/25/2023Addressing amino acid-derived inhibitory metabolites and enhancing CHO cell culture performance through DOE-guided media modificationsBiotechnology and Bioengineering***
3/1/2023Reconstruction of reverse transsulfuration pathway enables cysteine biosynthesis and enhances resilience to oxidative stress in Chinese Hamster Ovary cellsMetabolic Engineering***
12/1/2022Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturingBiotechnology and Bioengineering***
12/1/2021Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cellsMetabolic Engineering Communications***
11/13/2021A genome-scale nutrient minimization forecast algorithm for controlling essential amino acid levels in CHO cell culturesBiotechnology and Bioengineering***
8/31/2021Chemical speciation of trace metals in mammalian cell culture media: looking under the hood to boost cellular performance and product qualityCurrent Opinion in Biotechnology***
7/23/2019An unconventional uptake rate objective function approach enhances applicability of genome-scale models for mammalian cellsnpj Systems Biology and Applications***
5/29/2019Characterization of intact glycopeptides reveals the impact of culture media on site‐specific glycosylation of EPO‐Fc fusion protein generated by CHO‐GS cellsBiotechnology and Bioengineering***

Clemson University

DateArticleJournalPDF
7/1/2024Small-scale perfusion mimic cultures in the ambr250 HT bioreactor systemBiochemical Engineering Journal***
6/19/2024RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6β and WFS1 knockdown on fed-batch production of IgG1Scientific Reports***
5/1/2024Machine Learning and Deep Learning Strategies for Chinese Hamster Ovary Cell Bioprocess OptimizationFermentation***
2/10/2024In-line buffer exchange in the coupling of Protein A chromatography with weak cation exchange chromatography for the determination of charge variants of immunoglobulin G derived from chinese hamster ovary cell culturesJournal of Chromatography A***
12/1/2023Microevolutionary dynamics of eccDNA in Chinese hamster ovary cells grown in fed-batch cultures under control and lactate-stressed conditionsScientific Reports***
10/1/2023Minimally-disruptive method to estimate the volumetric oxygen mass transfer coefficient (kLa) for recombinant Escherichia coli fermentationsBiochemical Engineering Journal***
12/1/2022Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturingBiotechnology and Bioengineering***
11/7/2022Rapid metal speciation of cell culture media using reversed‐phase separations and inductively coupled plasma optical emission spectrometryBiotechnology Progress***
11/1/2022Sensitive real-time on-line estimator for oxygen transfer rates in fermentersJournal of Biotechnology***
7/20/2022Dynamics of Amino Acid Metabolism, Gene Expression, and Circulomics in a Recombinant Chinese Hamster Ovary Cell Line Adapted to Moderate and High Levels of Extracellular LactateGenes***
1/27/2022PID controls: the forgotten bioprocess parametersDiscover Chemical Engineering***
12/1/2021Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch culturesBMC Biotechnology***
11/4/2020Induced pluripotent stem cells can utilize lactate as a metabolic substrate to support proliferationBiotechnology Progress***

University of Delaware

DateArticleJournalPDF
6/19/2024RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6β and WFS1 knockdown on fed-batch production of IgG1Scientific Reports***
4/12/2024A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression systemBiotechnology Progress***
12/4/2023Prediction of CHO cell line stability using expression of DNA repair genesBiotechnology Journal***
7/21/2023Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturingBiotechnology and Bioengineering***
12/1/2022Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturingBiotechnology and Bioengineering***
5/16/2022Comprehensive assessment of host cell protein expression after extended culture and bioreactor production of CHO cell linesBiotechnology and Bioengineering***
2/14/2022Extracellular vesicles facilitate large-scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cellsBiotechnology and Bioengineering***
1/27/2022PID controls: the forgotten bioprocess parametersDiscover Chemical Engineering***
1/11/2022Characterization of Monoclonal Antibody Glycan Heterogeneity Using Hydrophilic Interaction Liquid Chromatography-Mass SpectrometryFrontiers in Bioengineering and Biotechnology***
12/1/2021Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch culturesBMC Biotechnology***
10/1/2021Chapter Thirteen - Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylationMethods in enzymology***
10/1/2021Analytical methods to characterize recombinant adeno-associated virus vectors and the benefit of standardization and reference materialsCurrent Opinion in Biotechnology***
12/1/2019The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problemCurrent Opinion in Biotechnology***
10/1/2019Host Cell Proteins During BiomanufacturingCurrent applications of cell culture engineering***
7/23/2019An unconventional uptake rate objective function approach enhances applicability of genome-scale models for mammalian cellsnpj Systems Biology and Applications***
9/22/2017Growth Rate Changes in CHO Host Cells Are Associated with Karyotypic HeterogeneityBiotechnology Journal***

University of Maryland

DateArticleJournalPDF
12/13/2023Detecting Features of Protein Structure through their Mediator-Accessible Redox ActivitiesBioRxiv***
8/29/2022Protein G: β-galactosidase fusion protein for multi-modal bioanalytical applicationsBiotechnology Progress***
6/18/2021A Redox-Based Autoinduction Strategy to Facilitate Expression of 5xCys-Tagged Proteins for ElectrobiofabricationFrontiers in Microbiology***
10/1/2020Electrochemical Dissolved Oxygen Sensor-Integrated Platform for Wireless In Situ Bioprocess MonitoringSensors and Actuators B: Chemical***
7/29/2020Transglutaminase-mediated assembly of multi-enzyme pathway onto TMV brush surfaces for synthesis of bacterial autoinducer-2Biofabrication***
6/12/2020Wireless Sensor-Integrated Platform for Localized Dissolved Oxygen Sensing in BioreactorsJournal of Microelectromechanical Systems***

University of Massachusetts Lowell

DateArticleJournalPDF
9/24/2024Driving towards digital biomanufacturing by CHO genome-scale modelsTrends in Biotechnology***
6/24/2024Metabolic engineering of Bcat1, Adh5 and Hahdb towards controlling metabolic inhibitors and improving performance in CHO cell-culturesBiochemical Engineering Journal***
3/21/2024Characterization of dynamic regulation in Chinese hamster ovary (CHO) cell cultures in the late exponential phaseBiochemical Engineering Journal***
2/7/2024Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturingBiotechnology Advances***
7/27/2023Trace metal optimization in CHO cell culture through statistical design of experimentsBiotechnology Progress***
7/21/2023Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturingBiotechnology and Bioengineering***
5/2/2023Transcriptomic features reveal molecular signatures associated with recombinant adeno-associated virus production in HEK293 cellsBiotechnology Progress***
5/1/2023Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturingBiotechnology and Bioengineering***
4/25/2023Addressing amino acid-derived inhibitory metabolites and enhancing CHO cell culture performance through DOE-guided media modificationsBiotechnology and Bioengineering***
12/1/2022Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturingBiotechnology and Bioengineering***
10/1/2022Modulation of nutrient precursors for controlling metabolic inhibitors by genome-scale flux balance analysisBiotechnology Progress***
12/1/2021Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cellsMetabolic Engineering Communications***
10/1/2021Adventitious agent detection methods in bio-pharmaceutical applications with a focus on viruses, bacteria, and mycoplasmaCurrent Opinion in Biotechnology***
9/1/2021Characterization of Chinese hamster ovary cell culture feed media precipitateBiotechnology Progress***
6/18/2021Effect of iron addition on mAb productivity and oxidative stress in Chinese hamster ovary cultureBiotechnology Progress***
1/1/2021n-Layer BET adsorption isotherm modeling for multimeric Protein A ligand and its lifetime determinationJournal of Chromatography B***
12/1/2020Bigdata analytics identifies metabolic inhibitors and promoters for productivity improvement and optimization of monoclonal antibody (mAb) production processBioresources and Bioprocessing***
9/1/2020Chromatography bioseparation technologies and in-silico modelings for continuous production of biotherapeuticsJournal of Chromatography A***
8/1/2020CHO cell productivity improvement by genome-scale modeling and pathway analysis: Application to feed supplementsBiochemical Engineering Journal***
7/1/2020Identifying metabolic features and engineering targets for productivity improvement in CHO cells by integrated transcriptomics and genome-scale metabolic modelBiochemical Engineering Journal***
5/1/2020At-line N-linked glycan profiling for monoclonal antibodies with advanced sample preparation and high-performance liquid chromatographyJournal of Bioscience and Bioengineering***
4/1/2020Bioprocess Technologies that Preserve the Quality of iPSCsTrends in Biotechnology***
4/1/2020A high-resolution measurement of nucleotide sugars by using ion-pair reverse chromatography and tandem columnsAnalytical and Bioanalytical Chemistry***
3/1/2020Integration of Time-Series Transcriptomic Data with Genome-Scale CHO Metabolic Models for mAb EngineeringProcesses***
8/30/2019Consequences of trace metal variability and supplementation on Chinese hamster ovary (CHO) cell culture performance: A review of key mechanisms and considerationsBiotechnology and Bioengineering***
3/1/2019The Current Scientific and Regulatory Landscape in Advancing Integrated Continuous Biopharmaceutical ManufacturingTrends in Biotechnology***