The University of Delaware brings to AMBIC expertise in genomic and proteomics,
metabolic flux analysis, as well as standardization issues.
- AMBIC Reference Cell, Product, Media, and Feeds
- Changes in Host Cell Protein During Extended Cell Culture
- CleanCHO: Targeted Elimination Of Secreted By-Products Of Amino Acid Catabolism To Improve Volumetric Productivity
- Controlling Glycosylation and Cell Performance by Inhibiting Genes for the Glycolytic Pathway
- Elucidating Amino Acid Metabolism in CHO Cells
- Establishing Markers For Stability And A Model For Accelerated Prediction Of Production (In)Stability
- Exploring The Space Of CHO Extracellular Vesicles & Their Potential Applications
- Integrated Model Of CHO Cell Growth, Substrate Uptake And Intracellular Metabolism For Process Design And Control
Facilities and Infrastructure
Much of the proposed activities will occur in either the Delaware Biotechnology Institute (DBI) or Colburn Laboratory (CLB).
- DBI is a 72,000 sq. ft. state of the art life science building with emergency backup power, a dedicated data center, shared core facilities, and investigator laboratories. The core facilities include those related to genomics and genome sequencing, bioinformatics and computational biology (Cathy Wu, director), proteomics and mass spectrometry (Kelvin Lee, director), flow cytometry (in Terry Papoutsakis’ laboratory), protein production and purification, and bioimaging. The bioimaging facility has several state of the art confocal microscopes, super resolution microscopes, TEM, SEM, AFM, and high-speed microscopes.
- CLB has shared use resources as well as investigator laboratories including shared cell culture spaces, laser scanning imagers, bioreactors, mass spectrometry for flux analysis and metabolites, and other relevant instrumentation.
Both DBI and CLB are situated within a short walk. Moreover, other state of the art shared analytical instrumentation is available in the newly opened 150,000 sq. ft. Harker Laboratory. These unique and highly specialized facilities that exist at UD offer unprecedented opportunity to impact AMBIC in a positive way and provide UD a special role within the overall AMBIC framework. Other details regarding available equipment and facilities is provided in the relevant section of this NSF proposal.