Current Projects
- Advanced Manufacturing Platform for Gene Therapeutic Production
- AMBIC Media Feeding Strategy Design and Testing via a Model-Predictive GUI
- AMBIC Reference Cell, Product, Media, Feeds
- Changes in Host Cell Protein During Extended Cell Culture
- Characterization of Media Precipitate and Determination of Equilibrium Constants and Precipitation Kinetics
- Characterizing Chemical Complexation and Speciation in order to Improve Medium and Feed Formulations
- CleanCHO: Targeted Elimination of Secreted By-Products of Amino Acid Catabolism to Improve Volumetric Productivity
- Controlling Glycosylation and Cell Performance by Inhibiting Genes for the Glycolytic Pathway
- Determining Genome Stability in CHO Cell Lineage
- Dipeptides in CHO culture: Elucidating Uptake Kinetics, Metabolism and Enhanced Solubility
- Elucidating Amino Acid Metabolism in CHO Cells
- Enhancing Media Solubility and Stability Predictions: Incorporating Hydrogen Bonding Effect and Chemical Complexation
- Enhancing Stability in CHO cells: The Linkages between Culture Stress, Chromatin, and Gene Expression
- Establishing Markers for Stability and a Model for Accelerated Prediction of Production (In)stability
- Exploring the Space of CHO Extracellular Vesicles & their Potential Applications
- Free Metal Ion Activity Determinations in AMBIC Media
- Identifying Inhibitory Waste By-Products in High Density CHO Cell Cultures
- Improving Process Understanding through Genome Scale Models and Metabolomics
- Increasing Genomic Stability and Antibody Titer Using Mammalian Artificial Chromosomes
- Integrated Model of CHO Cell Growth, Substrate Uptake and Intracellular Metabolism for Process Design and Control
- Metal Speciation in Cultures: Impact on Cell Growth, Productivity, and Quality
- Optimized In-Situ Surface Enhanced Raman Spectroscopy (SERS) for On-Line Monitoring of Cell Culture Metabolites
- Optimizing Nutrient Concentration Levels in Media and Feed Formulations
- ProductivityTesting the Targets: Validating the Best Spots for Targeted Insertion
- Quantifying Process Heterogeneity – ‘Smart Marbles’ Analytical Technologies
- Redox Speciation and Electrochemical Probing in AMBIC Media
- Understanding & Controlling ER Stress in High Secreting CHO Cells
- Understanding and Manipulating the Epigenome to Maximize CHO Cell Productivity
Over the past two decades, the biopharmaceutical industry has emerged as one of the major manufacturing industries of growth in the US and around the world. Biomanufacturing represents nearly 2% of the total US GDP (Carlson, 2011) and its fraction is growing (Glaser, 2013). Much of biomanufacturing involves the application of mammalian cells, especially Chinese hamster ovary (CHO) cells, to produce therapeutic proteins and other biologics, i.e. biopharmaceuticals. Our biomanufacturing research will focus on the upstream development of cell lines and production processes.
With the advent of wealth of new biological information brought about in large part through the revolutions in ‘omics and data quality, and manufacturing efficiencies. These changes provide an abundance of precompetitive research challenges for the biomanufacturing community in the upstream area to realize a future where biological knowledge can be harnessed to produce biologics more efficiently and effectively in a reliable and standardized framework.
Follow the current project links the see what we’ve been up to, or click the button below to see projects we’ve already completed!