2022-04 Develop an Inducible Stable Producer Cell Line for rAAV Production via Site-specific Integration
Project Description
Project ID: 2022-04
Design inducible circuits that can regulate the timing and level of gene expression for all components necessary to make rAAV
Develop an inducible stable producer cell line with site-specific integration for extensible rAAV production
Project Deliverables
- Refactor rAAV rep, cap, and helper genes using variations of the inducible Tet-off promoter
- Optimize the rAAV rep, cap, and helper gene expression levels for high titer rAAV, by Kozak sequences and degrons
- Establish an inducible stable producer cell line with site specific integration in well-known human genomic safe harbors for rAAV production
| Project Leaders | Seongkyu Yoon (PI) / Mark Blenner (Co-PI) |
|---|---|
| University | University of Delaware and University of Massachusetts, Lowell |
Mentor Meetings
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